MiR-124 Regulates the Inflammation and Apoptosis in Myocardial Infarction Rats by Targeting STAT3.
作者： Ben-Qiang Xin , Lei Li , Xiao-Jing Cheng
DOI: 10.1007/S12012-021-09661-2
关键词:
摘要: This study aimed to discover the effect of miR-124/STAT3 axis on inflammation and cell apoptosis in myocardial infarction (MI) rats. Sprague-Dawley (SD) male rats were selected for establishing MI models divided into Sham, MI, + anti-miR-124 Ad-miR-124 groups. Cardiac function was detected via echocardiography. Hematoxylin & eosin (HE) triphenyltetrazolium chloride (TTC) staining used observe pathological changes area, while transferase (TdT)-mediated D-UTP-biotin nick end labeling (TUNEL) assay apoptosis. Enzyme-linked immunosorbent (ELISA) determine serum levels inflammatory cytokines. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) western blotting performed mRNA protein levels, respectively. Dual luciferase reporter gene revealed that STAT3 a target miR-124. The expression miR-124 increased pSTAT3/STAT3 ratio reduced group showed enhanced LVEDD LVESD, LVEF LVFS, as well larger area compared with Sham group, Besides, IL-1β, IL-6, TNF-α MCP-1 elevated expressions Bax/Bcl-2 cleaved caspase-3 downregulated group. We further found silencing improved cardiac function, cytokines, prevented Silencing could inhibit cells, thereby relieving injury upregulation STAT3.
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