E-beam irradiation & steam explosion as biomass pretreatment, and the complex role of lignin in substrate recalcitrance
作者： Timo Leskinen , Stephen S. Kelley , Dimitris S. Argyropoulos
DOI: 10.1016/J.BIOMBIOE.2017.05.008
关键词:
摘要: Abstract Sequential electron beam-steam explosion (EB-SE) pretreatment was applied to hardwood (Birch) and softwood (Pine) substrates in an effort enhance their enzymatic saccharification. The effect of these two pretreatments on the structure composition individual cell wall components examined. combination treatments showed a synergistic conversion hemicelluloses into water soluble oligomers, enhanced overall saccharification wood substrates. Even after combined Pine more recalcitrant than Birch, which attributed differences lignin. Model systems created from cellulose isolated high molecular weight (HMW) lignin fractions were found inhibit by 20% over control. This inhibition is likely related unproductive binding enzymes HMW Additionally, presence reduces swelling capacity substrate, thus its accessibility enzymes. Conversely, low fragments be slightly beneficial for hydrolysis These results provide insight complex interactions between cellulase enzymes, highlight need processes that can effectively cleave oligomeric fragments.
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