Culture of the Mouse Preimplantation Embryo

摘要: The first culture media designed specifically to support development of the preimplantation mouse embryo were formulated over 50 years ago and based on balanced salt solutions, containing carbohydrates pyruvate, lactate, glucose as sole energy sources. Such used a bicarbonate-carbon dioxide buffer system maintain desired pH, typically supplemented with serum albumin, but lacked free amino acids. In contrast complexity tissue medium, these original formulations very simplistic. Over intervening decades, our understanding physiology metabolism increased, together greater environment within female reproductive tract, in vitro have become more physiological consequently complex. A main addition such has been an array Although today contain components than their predecessors, preparation remains relatively easy accomplish, made feasible through use stock which also readily facilitates any changes be made, essential prerequisite for experimentation. As well formulations, there exciting developments incubator technology design, inclusion time-lapse capability, redefining ability both monitor vitro.