Triazole-linked DNA as a primer surrogate in the synthesis of first-strand cDNA.
作者： Tomoko Fujino , Ken-ichi Yasumoto , Naomi Yamazaki , Ai Hasome , Kazuhiro Sogawa
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摘要: A phosphate-eliminated nonnatural oligonucleotide serves as a primer surrogate in reverse transcription reaction of mRNA. Despite the triazole linkages surrogate, transcriptase effectively elongated cDNA sequences on 3'-downstream by complementary sequence structure-activity comparison with reference natural oligonucleotides shows superior priming activity containing triazole-linkages. The also protect transcribed from digestion reactions 5'-exonuclease and enable us to remove noise transcripts unknown origins.
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