Invertase stability in alginate beads: Effect of trehalose and chitosan inclusion and of drying methods

摘要: Abstract The purpose of present work was to analyze the effect trehalose and chitosan on invertase activity preservation in wet dried alginate beads, by taking advantage well known protective labile compounds during drying improvement mechanical properties beads. beads were subjected different methods (freeze-drying, vacuum forced air circulation drying) a mild thermal treatment (50 °C). size morphology analyzed optical microscopy scanning electron microscopy. affected at an extent which depended more method than composition. Freeze-drying allowed better maintenance diameter circularity showed smoother surface smaller pores those generated or drying. inclusion gelation media improved enzymatic recovery after generation; this fact attributed higher resistance provided interaction between chitosan. There no step. Interactions chitosan, as presence manifested FT-IR spectra, increase decomposition temperatures detected DSC. In not enough protected from damage caused heat treatment. stability independent type employed, being mainly Although effective prevent enzyme functionality generation, it essential for achieving adequate protection freezing, (freeze-,