Phenolics Isolated from Aframomum meleguta Enhance Proliferation and Ossification Markers in Bone Cells
作者： Ashraf Abdel-Naim , Abdullah Alghamdi , Mardi Algandaby , Fahad Al-Abbasi , Ahmed Al-Abd
DOI: 10.3390/MOLECULES22091467
关键词:
摘要: Osteoporosis is a serious health problem characterized by decreased bone mineral density and deterioration of microarchitecture. Current antiosteoporotic agents exhibit wide range adverse effects; meanwhile, phytochemicals are effective safer alternatives. In the current work, nine compounds belonging to hydroxyphenylalkane diarylheptanoid groups were isolated from Aframomum meleguea seeds identified as 6-gingerol (1), 6-paradol (2), 8-dehydrogingerdione (3), 8-gingerol (4), dihydro-6-paradol (5), dihydrogingerenone A (6), C (7), 1,7-bis(3,4-dihydroxy-5-methoxyphenyl)heptane-3,5-diyl diacetate (8), 1-(3,4-dihydroxy-5-methoxyphenyl)-7-(3,4-dihydroxyphenyl)heptane-3,5-diyl (9). The structures established NMR mass spectral data, in addition referring literature data. Exposure MCF-7, MG-63, SAOS-2 cells subcytotoxic concentrations under investigation resulted accelerated proliferation. Among them, paradol was selected for further detailed biochemical analysis cells. DNA flowcytometric cell cycle distribution revealed that did not induce any significant change proliferation index Assessment osteogenic gene expression enhanced osteocyte osteoblast-related genes inhibited osteoclast RUNX suppressor genes. Biochemically, alkaline phosphatase activity vitamin D content osteoporotic marker acid phosphatase. conclusion, paradol, which major constituents A. melegueta seeds, exhibited potent proliferative ossification characteristics
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