A modified method of insulin producing cells' generation from bone marrow-derived mesenchymal stem cells.

摘要: Type 1 diabetes mellitus is a result of autoimmune destruction pancreatic insulin producing β-cells and so far it can be cured only by injection, pancreas transplantation, or islet cells’ transplantation. The methods are, however, imperfect have lot disadvantages. Therefore new solutions are needed. best one would the use differentiated mesenchymal stem cells (MSCs). In present study, we investigated potential bone marrow-derived MSCs line for in vitro differentiation into (IPSs). We applied an 18-day protocol to differentiate MSCs. Differentiating formed cell clusters some which resembled islet-like cells. Using dithizone confirmed presence What more, expression proinsulin C-peptide IPCs was analyzed flow cytometry. For first time, influence growth factors’ concentration on efficiency. found that increase factors up 60 ng/mL β-FGF/EGF 30 ng/mL activin A/β-cellulin increases percentage IPCs. Further does not show any Our findings suggest presented adapted from