MicroRNAs Classify Different Disease Behavior Phenotypes of Crohn's Disease and May Have Prognostic Utility.

摘要: There is a dire need for reliable prognostic markers that can guide effective therapeutic intervention in Crohn's disease (CD). We examined whether different phenotypes CD be classified based on colonic microRNA (miRNA) expression and miRNAs have utility CD. Methods: High-throughput sequencing of small total RNA isolated from colon tissue patients with controls without Inflammatory Bowel Disease (non-IBD) was performed. To identify associated specific CD, were stratified according to behavior (nonstricturing, nonpenetrating; stricturing; penetrating), miRNA profiles each subset compared those the non-IBD group. Validation assays performed using quantitative reverse transcription polymerase chain reaction. These further evaluated by transcriptase reaction formalin-fixed, paraffin-embedded (index biopsies) nonpenetrating at time diagnosis either retained phenotype or progressed penetrating/fistulizing CD. Results: We found suite miRNAs, including miR-31-5p, miR-215, miR-223-3p, miR-196b-5p, miR-203 stratify independent effect inflammation. Furthermore, we also demonstrated levels miR-215 index biopsies might predict likelihood progression CD. Finally, novel statistical simulation approach applied RNA-sequencing data controls, identified miR-31-5p as candidate master regulators gene CD. Conclusions: miRNAs may serve clinically useful guiding initial therapy identifying who would benefit most intervention.