Suppression of Staphylococcus aureus biofilm formation and virulence by a benzimidazole derivative, UM-C162.

摘要: Staphylococcus aureus is a major cause of nosocomial infections and secretes diverse spectrum virulence determinants as well forms biofilm. The emergence antibiotic-resistant S. highlights the need for alternative therapeutics other than conventional antibiotics. One route to meet this screening small molecule derivatives potential anti-infective activity. Using previously optimized C. elegans - screen, we identified benzimidazole derivative, UM-C162, which rescued nematodes from infection. UM-C162 prevented formation biofilm in dose-dependent manner without interfering with bacterial viability. To examine effect on expression genes, genome-wide transcriptome analysis was performed UM-C162-treated pathogen. Our data indicated that genes associated formation, particularly those involved attachment, were suppressed bacteria. Additionally, set encoding vital factors also down-regulated presence UM-C162. Further biochemical validated UM-C162-mediated disruption hemolysins, proteases clumping production. Collectively, our findings propose promising compound can be further developed an anti-virulence agent control infections.