Single molecule identification using selected fluorescence characteristics
作者： James H. Jett , Peter Goodwin , Richard A. Keller , Nicholas P. Machara , Orden Alan K. Van
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摘要: Single fluorescent molecules in a flowing sample stream (14) are distinguished and identified using only single laser excitation wavelength (38). A is formed containing dilute mixture of molecule fluorophores (12), wherein each one the serially ordered (14). The illuminated with (38) effective to excite fluorophore at time. Fluorescence emission photons from said detected (48). burst size determined for identify fluorophore. pulsed may be used (38), where an intra-burst fluorescence decay rate simultaneously photons. correlated
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