Ultrastructural localization of acidic glycoconjugates with the low iron diamine method.

摘要: The present study has applied the low iron diamine (LID) method at ultrastructural level to demonstrate acid glycoconjugates. We have examined rat epiphyseal cartilage, human bone marrow, tracheal glands, and mouse sublingual glands stained with LID prior embedment. staining appeared require postosmication for adequate visualization electron microscope level. Thiocarbohydrazide-silver proteinate (TCH-SP) of thin sections variably enhanced reactive sites. LID-TCH-SP carboxyl sulfate groups glycosaminoglycans in extracellular cartilage matrix, secretory granules, expanded Golgi saccules chondrocytes. In cytoplasmic known contain sulfated glycosaminoglycans, external surface plasma membrane leukocytes. Moderately strong was observed granules mucous tubules glycoproteins, acinar cells a sialoglycoprotein. lack glycoconjugates gland confirmed by their failure stain high method. Thus these studies indicate that methods are useful localization carboxylated intracellular