Determination and physiological roles of the glycosylphosphatidylinositol lipid remodelling pathway in yeast

摘要: Summary In the yeast Saccharomyces cerevisiae, glycosylphosphatidylinositol (GPI)-anchored proteins play important roles in cell wall biogenesis/assembly and formation of lipid microdomains. The moieties mature GPI-anchored typically contain either ceramide or diacylglycerol. Recent studies have identified that GPI phospholipase A2 Per1p O-acyltransferase Gup1p essential diacylglycerol-type remodelling proteins, while Cwh43p is involved to ceramide. It has been generally proposed phosphatidylinositol with diacylglycerol containing a C26 saturated fatty acid, which generated by sequential activity Gup1p, converted inositolphosphorylceramide Cwh43p. In this report, we constructed double-mutant strains defective investigated their growth phenotypes proteins. Based on our analyses single- double-mutants remodelling, demonstrate an alternative pathway, lyso-phosphatidylinositol used as substrate for Cwh43p, when normal pathway inhibited. addition, mass spectrometric analysis species Flag-tagged Gas1p revealed contains its anchor.