In Vitro Employment of Recombinant Taenia solium Calreticulin as a Novel Strategy Against Breast and Ovarian Cancer Stem-like Cells.

摘要: Background and Aims Calreticulin is a chaperone master regulator of intracellular calcium homeostasis. Several additional functions have been discovered. Human parasite calreticulin shown to suppress mammary tumor growth in vivo. Here, we explored the capacity recombinant Taenia solium (rTsCRT) modulate cancer cell in vitro. Methods We used different concentrations rTsCRT treat lines analyzed viability colony formation capacity. also tested combination IC20 or IC50 doses chemotherapeutic drug 5-fluorouracil on MCF7 SKOV3 lines. As control, non-tumorigenic line MCF10-A was employed. The effect combinations assessed in stem-like cells. Additionally, scavenger receptor ligands were employed identify role this anti-tumoral effect. Results has dose-dependent in vitro effect, being most sensitive followed by MCF7. When rTsCRT/5-fluorouracil used, showed 60% reduction viability; diminished. Treatment cells from higher viability, while those more disaggregation. Finally, pharmacological inhibition receptor, abrogated induced both parental Conclusion Our data suggest that alone with inhibits breast ovarian through its interaction receptors.