AT1R blockade reduces IFN-γ production in lymphocytes in vivo and in vitro

摘要: AT 1 R blockade reduces IFN-γ production in lymphocytes vivo and vitro. Background Type angiotensin II (Ang II) receptor (AT R) signaling induces proinflammatory responses. Recent studies suggest that T express R; yet the effects of Ang binding to on cells are poorly understood. We examined effect release cytokine, interferon-gamma (IFN-γ) by human Methods used an blocker losartan a randomized clinical trial kidney transplant recipients over 12-month period [AT ( N = 11) control 10)]. Peripheral blood lymphocytes, isolated from both cohorts, were analyzed enzyme-linked immunosorbent spot assays (ELISPOT) analyses real-time reverse transcription-polymerase chain reaction (RT-PCR) enumerate producing mRNA levels. The vitro assessed using alloreactive cytotoxic T-lymphocyte line. Alloreactive treated with or candesartan immunosorbant assay (ELISA) was measure protein release. line also blocker–treated prior determining intracellular cytokine staining. Results cohort had significant decrease peripheral P ≤ 0.05 for each time point) levels 0.01 vs. patients). Losartan decreased treatment inhibited II's furthermore Conclusion blockers may have clinically relevant immunomodulatory role blocking cells.