Identification of AML-1 and the (8;21) translocation protein (AML-1/ETO) as sequence-specific DNA-binding proteins: the runt homology domain is required for DNA binding and protein-protein interactions.

作者: S Meyers , J R Downing , S W Hiebert

DOI: 10.1128/MCB.13.10.6336

关键词:

摘要: The AML1 gene on chromosome 21 is disrupted in the (8;21)(q22;q22) translocation associated with acute myelogenous leukemia and encodes a protein central 118-amino-acid domain 69% homology to Drosophila pair-rule gene, runt. We demonstrate that AML-1 DNA-binding which specifically interacts sequence belonging group of enhancer core motifs, TGT/cGGT. Electrophoretic mobility shift analysis cell extracts identified two AML-1-containing protein-DNA complexes whose electrophoretic mobilities were slower than those formed produced vitro. Mixing vitro-produced prior gel resulted formation higher-order complexes. Deletion mutagenesis revealed runt mediates both sequence-specific DNA binding protein-protein interactions. hybrid product, AML-1/ETO, results from (8;21) retains domain, recognizes consensus other cellular proteins.

参考文章(37)
A Thornell, B Hallberg, T Grundström, Binding of SL3-3 enhancer factor 1 transcriptional activators to viral and chromosomal enhancer sequences. Journal of Virology. ,vol. 65, pp. 42- 50 ,(1991) , 10.1128/JVI.65.1.42-50.1991
S W Hiebert, M Blake, J Azizkhan, J R Nevins, Role of E2F transcription factor in E1A-mediated trans activation of cellular genes. Journal of Virology. ,vol. 65, pp. 3547- 3552 ,(1991) , 10.1128/JVI.65.7.3547-3552.1991
J M Redondo, J L Pfohl, C Hernandez-Munain, S Wang, N A Speck, M S Krangel, Indistinguishable nuclear factor binding to functional core sites of the T-cell receptor delta and murine leukemia virus enhancers. Molecular and Cellular Biology. ,vol. 12, pp. 4817- 4823 ,(1992) , 10.1128/MCB.12.11.4817
Y Kamachi, E Ogawa, M Asano, S Ishida, Y Murakami, M Satake, Y Ito, K Shigesada, Purification of a mouse nuclear factor that binds to both the A and B cores of the polyomavirus enhancer. Journal of Virology. ,vol. 64, pp. 4808- 4819 ,(1990) , 10.1128/JVI.64.10.4808-4819.1990
H Weiher, M Konig, P Gruss, Multiple point mutations affecting the simian virus 40 enhancer. Science. ,vol. 219, pp. 626- 631 ,(1983) , 10.1126/SCIENCE.6297005
James Vardiman, Daniel Catovsky, George R. Flandrin, Robert Pierre, Correlation Between Morphology and Karotype Cancer Genetics and Cytogenetics. ,vol. 11, pp. 275- 281 ,(1984) , 10.1016/S0165-4608(84)80005-9
Steffen Faisst, Silke Meyer, Compilation of vertebrate-encoded transcription factors. Nucleic Acids Research. ,vol. 20, pp. 3- 26 ,(1992) , 10.1093/NAR/20.1.3