Speciated human high-density lipoprotein protein proximity profiles.

摘要: It is expected that the attendant structural heterogeneity of human high-density lipoprotein (HDL) complexes a determinant its varied metabolic functions. To determine HDL, we determined major apolipoprotein stoichiometry profiles in HDL. First, HDL was separated into two main populations, with and without (apo) A-II, LpA-I LpA-I/A-II, respectively. Each population further six individual subfractions using size exclusion chromatography (SEC). Protein proximity (PPPs) apolipoproteins each subfraction by optimally cross-linking within particles bis(sulfosuccinimidyl) suberate (BS3), bifunctional cross-linker, followed molecular mass determination MALDI-MS. The PPPs indicated number apoA-I molecules increased from to three four an increase particle size. On other hand, en...