Flow cytometric cell cycle analysis using the quenching of 33258 Hoechst fluorescence by bromodeoxyuridine incorporation.
作者： R. -M. Bohmer
DOI: 10.1111/J.1365-2184.1979.TB00117.X
关键词:
摘要: Cultures of L cells were grown in medium containing 2.0 mg/l bromodeoxyuridine (BUdR) and stained with the fluorescent dye 33258 Hoechst for flow cytometric analysis. During exposure to BUdR, The replace thymidine by BUdR newly synthesized DNA. new DNA is not stainable Hoechst, which highly specific thymidine. temporal development fluorescence distributions after addition growth has been investigated cytometer, data used calculate mean durations phases G1, S G2 + M exponentially growing cultures as well cycle transit times synchronized cultures. percentage non-cycling was determined each experiment.
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