Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease

摘要: Biomarkers for the detection of pre-cancer and cancer cells a method their at point care are disclosed. The system is comprised an inexpensive, disposable microfluidic cassette sample preparation; continuous-flow, magnetic-bead assisted cell sorting cytometry; lysis; nucleic acid isolation amplification, hybridization, quantification, analysis. Typically, will be used with benchtop instrument platform that provides power, control capabilities to cassette. In one particular embodiment application invention, based on selective binding magnetic beads coated MCAP antibody exfoliated precancerous cancerous contained in saliva. bead-labeled then separated from other saliva components using field. Both labeled unlabeled can counted integrated detectors several types, including counters impedance measurements, cytometers Coulter particle counter principles. sorted sub-population lysed, acids isolated gene assays and/or transcription profiling subpopulation. includes re verse- polymerase chain reaction hybridization array identification. devices, systems, modes operation, methods disclosed here also applicable disease body fluids.