Numerical simulation of column performance in ion-exclusion chromatography

摘要: Abstract The separation mechanism of acidic compounds in ion-exclusion chromatography has been described using two approaches. first one is based on the global thermodynamic and chromatographic relationships, other a computer simulation column performance. Strong acids leave unseparated, within its dead volume, weak appear at end chromatogram also unseparated. It was found that addition an compound eliminates dependence retention volume sample concentration. By use simulations, evolution peaks with time peak shape for analyzed have studied. results compare favourably experimental data available literature.