Efficient transfection of the archaebacterium Halobacterium halobium.

摘要: We developed an efficient polyethylene glycol-mediated spheroplast transfection method for the extremely halophilic archaebacterium Halobacterium halobium. The 59-kilobase-pair linear phage phi H DNA molecule routinely produced between 5 X 10(6) and 2 10(7) transfectants per microgram of DNA. Between 0.5 1% spheroplasts were transfected luminal diameter Under our conditions, survival regeneration H. halobium also quite efficient, suggesting that this will be useful introducing other DNAs into these bacteria.