Observations on nucleic acid and polyphosphate in Torulopsis utilis.

摘要: Abstract 1. The nucleic acid fraction of yeast as obtained by the Schneider technique contains an additional phosphorus compound; it is suggested that a linear polyphosphate high molecular weight. 2. This has necessitated development modified determination for whereby phosphate and are determined in presence each other. method evolved consists differential hydrolysis 5% trichloracetic at 90°C 1 hour. rate two phosphates been determined. 3. By application technique, behaviour components followed throughout growth cycle. It shown total amount decreases during early active phase, then increases rapidly, increasing even after protein synthesis finished. Fluctuation ratio UV absorption to NAP, this method, composition itself may vary cycle; highest values were termination synthesis, lowest growth. significance these observations discussed. 4. Studies have made on samples which free from polyphosphate. evidence regarding P investigations those T. utilis discussed with respect structure acid. 5. demonstrated metachromatic reaction toluidine blue given number polyanions not restricted “hexametaphosphate”. nomenclature substance