Synthetic mRNA devices that detect endogenous proteins and distinguish mammalian cells.
作者： Shunsuke Kawasaki , Yoshihiko Fujita , Takashi Nagaike , Kozo Tomita , Hirohide Saito
DOI: 10.1093/NAR/GKX298
关键词:
摘要: Synthetic biology has great potential for future therapeutic applications including autonomous cell programming through the detection of protein signals and production desired outputs. RNA devices are promising this purpose. However, number available is limited due to difficulty in endogenous proteins within a cell. Here, we show strategy construct synthetic mRNA that detect living cells, control translation distinguish types. We engineered protein-binding aptamers have increased stability secondary structures their active conformation. The designed can efficiently respond target human LIN28A U1A proteins, while original failed do so. Moreover, delivery an LIN28A-responsive device into induced pluripotent stem cells (hiPSCs) revealed hiPSCs differentiated by quantifying expression level. Thus, our protein-driven determine live-cell states program mammalian based on intracellular information.
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