Microbial Diversity and Resistance to Copper in Metal-Contaminated Lake Sediment

摘要: Contamination of habitats with heavy metals has become a worldwide problem. We describe herein the analysis lake sediment contaminated high concentrations copper as consequence mine milling disposal over 100-year period. Copper in were found to vary depth and ranged from 200 5500 ppm. Analysis microbial community T-RFLP identified minimum 20 operational taxonomic units (OTU). along profile detected many nine shared OTUs across 15 centimeters, suggesting conservation structure this range. Only two genera, Arthrobacter Ralstonia, among 50 aerobic copper-resistant isolates cultivated on R2A, one which (Ralstonia sp.) was characterized by sequestration copper, electron diffraction scanning, growing colonies. Scanning microscopy showed changes outer envelope cells when grown presence copper. The Ralstonia also resistant Ni, Cd, Zn, showing patterns phenotypic these three either resistance Zn or Ni expressed an isolate but never both.