Bioinformatic analysis of epithelial:mesenchymal crosstalk during mouse gut development and patterning

摘要: Reciprocal crosstalk between the endodermally-derived epithelium and underlying mesenchyme is required for regional patterning proper differentiation of developing mammalian intestine. Though both participate in patterning, thought to play a prominent role determination epithelial phenotype during development adult life. However, molecular basis this instructional dominance unclear. In fact, surprisingly little known about cellular origins many critical signaling molecules gene transcriptional events that they impact. Here, we profile genes are expressed separate mesenchymal compartments perinatal mouse The data indicate vast majority soluble inhibitors modulators pathways such as Hedgehog, Bmp, Wnt, Fgf Igf predominantly or exclusively by mesenchyme, accounting its ability dominate crosstalk. We also catalog most highly enriched transcription factors compartments. results bolster previous evidence suggesting major Hnf4α Hnf4γ regulation genes. Finally, find while epithelially 69 tend be tissue-restricted their expression, mesenchymally broadly multiple tissues. Thus, unique tissue-specific signature characterizes intestinal instructed supported itself expresses largely non-tissue specific.