Applications of flow cytometry for measurement of autophagy
作者： Alik Demishtein , Ziv Porat , Zvulun Elazar , Elena Shvets
DOI: 10.1016/J.YMETH.2014.12.020
关键词:
摘要: Autophagy is a dynamic catabolic process that plays major role in sequestering and recycling cellular components multiple physiological pathophysiological conditions. Despite recent progress our understanding of the autophagic there still shortage robust methods for monitoring autophagy live cells. Flow cytometry, reliable unbiased method quantitative collection data high-throughput manner, was recently utilized to monitor activity fixed mammalian In this article we summarize advantages potential pitfalls use flow cytometry study autophagy.
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sci-hub.se 参考文章(48)
Joseph E. Coyle, Seema Qamar, Kanagalaghatta R. Rajashankar, Dimitar B. Nikolov, Structure of GABARAP in Two Conformations Neuron. ,vol. 33, pp. 63- 74 ,(2002) , 10.1016/S0896-6273(01)00558-X
Ewa K. Zuba-Surma, Mariusz Z. Ratajczak, Analytical capabilities of the ImageStream cytometer. Methods in Cell Biology. ,vol. 102, pp. 207- 230 ,(2011) , 10.1016/B978-0-12-374912-3.00008-0
Russell B. Wilson, Robert F. Murphy, Chapter 16 Flow-Cytometric Analysis of Endocytic Compartments Methods in Cell Biology Volume 31. ,vol. 31, pp. 293- 317 ,(1989) , 10.1016/S0091-679X(08)61616-7
Claire de la Calle, Pierre-Emmanuel Joubert, Helen K.W. Law, Milena Hasan, Matthew Albert, Simultaneous assessment of autophagy and apoptosis using multispectral imaging cytometry. Autophagy. ,vol. 7, pp. 1045- 1051 ,(2011) , 10.4161/AUTO.7.9.16252
F Ann Ran, Patrick D Hsu, Jason Wright, Vineeta Agarwala, David A Scott, Feng Zhang, Genome engineering using the CRISPR-Cas9 system Nature Protocols. ,vol. 8, pp. 2281- 2308 ,(2013) , 10.1038/NPROT.2013.143
Yukiko Kabeya, Noboru Mizushima, Takashi Ueno, Akitsugu Yamamoto, Takayoshi Kirisako, Takeshi Noda, Eiki Kominami, Yoshinori Ohsumi, Tamotsu Yoshimori, LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing The EMBO Journal. ,vol. 19, pp. 5720- 5728 ,(2000) , 10.1093/EMBOJ/19.21.5720
Ezgi Tasdemir, M. Chiara Maiuri, Nicolas Tajeddine, Ilio Vitale, Alfredo Criollo, José Miguel Vicencio, John A. Hickman, Olivier Geneste, Guido Kroemer, Cell cycle-dependent induction of autophagy, mitophagy and reticulophagy. Cell Cycle. ,vol. 6, pp. 2263- 2267 ,(2007) , 10.4161/CC.6.18.4681
Natalia von Muhlinen, Masato Akutsu, Benjamin J. Ravenhill, Ágnes Foeglein, Stuart Bloor, Trevor J. Rutherford, Stefan M.V. Freund, David Komander, Felix Randow, LC3C, bound selectively by a noncanonical LIR motif in NDP52, is required for antibacterial autophagy Molecular Cell. ,vol. 48, pp. 329- 342 ,(2012) , 10.1016/J.MOLCEL.2012.08.024
Hitoshi Nakatogawa, Kuninori Suzuki, Yoshiaki Kamada, Yoshinori Ohsumi, Dynamics and diversity in autophagy mechanisms: lessons from yeast Nature Reviews Molecular Cell Biology. ,vol. 10, pp. 458- 467 ,(2009) , 10.1038/NRM2708
Vladimir Kirkin, Trond Lamark, Yu-Shin Sou, Geir Bjørkøy, Jennifer L. Nunn, Jack-Ansgar Bruun, Elena Shvets, David G. McEwan, Terje H. Clausen, Philipp Wild, Ivana Bilusic, Jean-Philippe Theurillat, Aud Øvervatn, Tetsuro Ishii, Zvulun Elazar, Masaaki Komatsu, Ivan Dikic, Terje Johansen, A Role for NBR1 in Autophagosomal Degradation of Ubiquitinated Substrates Molecular Cell. ,vol. 33, pp. 505- 516 ,(2009) , 10.1016/J.MOLCEL.2009.01.020