Assay for Ribozyme target site
作者： James A. McSwiggen
DOI:
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摘要: Method for determining target site accessibility a ribozyme by providing DNA oligonucleotide having nucleotide sequence complementary to the RNA target. The is contacted with in presence of an agent, such as RNAseH, that RNAseH cleaves when and form duplex. occurrence cleavage detected any standard methodology. Those oligonucleotides which provide detectable level indicate accessible ribozyme.
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