Fibroblasts from idiopathic pulmonary fibrosis and normal lungs differ in growth rate, apoptosis, and tissue inhibitor of metalloproteinases expression.
作者： Carlos Ramos , Martha Montaño , Jorge Garcı́a-Alvarez , Vı́ctor Ruiz , Bruce D. Uhal
关键词:
摘要: Idiopathic pulmonary fibrosis (IPF) is a chronic lung disorder characterized by fibroblast proliferation and extracellular matrix accumulation. However, studies on growth rate collagen synthesis have given contradictory results. Here we analyzed formazan-based chromogenic assay; apoptosis in situ end labeling (ISEL) propidium iodide staining; percent of α -smooth muscle actin ( -SMA) positive cells fluorescence-activated cell sorter; 1-(I) collagen, transforming factor (TGF)- β 1, collagenase-1, gelatinases A B, tissue inhibitor metalloproteinase (TIMP)-1, -2, -3, -4 expression reverse transcriptase/polymerase chain reaction fibroblasts derived from IPF control lungs. Growth was significantly lower compared with controls (13.3 ± 38.5% versus 294.6 57%, P < 0.0001 at 13 d). Conversely, higher percentage apoptotic observed IPF-derived (...
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