Clonal diversity in a Rhododendron ferrugineum L. (Ericaceae) population inferred from AFLP markers

作者: N. ESCARAVAGE , S. QUESTIAU , A. PORNON , B. DOCHE , P. TABERLET

DOI: 10.1046/J.1365-294X.1998.00415.X

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摘要: In the European Alps, Rhonodendron ferrugineum can constitute dense populations with almost 100% of cover. The developmental pattern by layering and resulting complexity population structure make it difficult to identify distinct clones even excavation. Therefore genotypic a R. population, in French was inferred from AFLP markers. first step, we analysed 400 samples using profiles generated one selective primer pair. Seventeen bands out 25 were polymorphic (68%). We identified total 32 multilocus genotypes. second genotypes verified applying two additional pairs most distant each genotype. mean similarity (proportion band sharing) between 0.85 (range 0.52 0.94). spatial distribution showed that vegetative spreading mainly occurred down slope. Based on an annual shoot growth 2.6 cm/year size widest clone, estimated age oldest individual be at least 300 years. A single genotype occupy large surface sometimes form patch, suggesting this species adopts phalanx limited intermingling some genets.

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