Development of bronchus-associated lymphoid tissue hyperplasia following lipopolysaccharide-induced lung inflammation in rats.

摘要: Gram-negative bacterial endotoxin lipopolysaccharide (LPS) administration has been used as an animal model of sepsis-related acute lung injury and adult respiratory distress syndrome (ALI/ARDS). This paper describes the histology following induced by intraperitoneal (i.p.) to rats, in comparison with earlier findings. ALI was i.p. Esherichia coli LPS 2 (n = 8) or 3 5) mg/kg, whereas physiological saline administered control animals 5). Eighteen hours after injections, were euthanized. The lungs heart removed one block for histological study (hematoxylin eosin [HE light microscopy). tissue (bronchial wall, vessels, alveoli, interstitium) graded via a scoring system (0 3+). showed intact tissue. Ten 13 group had bronchus-associated lymphoid (BALT) hyperplasia. Pathological signs ALI/ARDS, diffuse alveolar damage (DAD) emphysema, observed 5 8 cases, respectively. injection induces primarily BALT hyperplasia also less characteristic DAD. rat is suitable investigation not only ALI/ARDS but occurring consequence chronic pulmonary inflammatory processes.