Protective effect of vanadate on oxyradical-induced changes in isolated perfused heart.

摘要: In order to examine the mechanisms of beneficial effects vanadate on cardiac dysfunction in chronic diabetes, rat hearts were perfused with xanthine plus oxidase, an oxyradical generating system absence or presence vanadate. The heart failed generate contractile force and increased resting tension markedly within 5 min perfusion oxidase. These changes prevented by addition 4 μM medium. protective loss developed due oxidase dose-dependent (0.1–5 μM). Perfusion glucose-free medium did not abolish actions sarcolemmal Ca2+-pump (ATP-dependent Ca2+ uptake Ca2+-stimulated ATPase) Na+-dependent activities decreased upon perfusing a containing for min; these 2–4 signals superoxide radicals produced as detected electron paramagnetic resonance spectroscopic technique, inhibited 5–100 results suggest that is scavenger thus may prevent under some pathological conditions its antioxidant action.