Quantitative protein analysis by immunodiffusion

作者: Frederick J. Aladjem , Padmasini K. Ayengar

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摘要: Methods and apparatus are described by which immunochemical procedures such as immunodiffusion immunoelectrophoresis can be used to provide quantitative measurement of the concentration individual proteins in fluids serum, spinal fluid, tissue extracts like. That is accomplished typically photooptically scanning zones precipitation produced, parallel with those standard preparations containing known concentrations determined; deriving from arrays measurements selected parameters; comparing parameter values experimental reference preparations. The zone preferably made recorded electronically, suitable digital manipulation resulting video at a plurality positions for each zone.