Phosphatidylinositol 3-kinase mediates epidermal growth factor-induced activation of the c-Jun N-terminal kinase signaling pathway.

摘要: The signaling events which mediate activation of c-Jun N-terminal kinase (JNK) are not yet well characterized. To broaden our understanding upstream mediators link extracellular signals to the JNK pathway, we investigated role phosphatidylinositol (PI) 3-kinase in epidermal growth factor (EGF)-mediated activation. In this report demonstrate that a dominant negative form PI as inhibitor wortmannin blocks EGF-induced dramatically. However, does have an effect on induced by UV irradiation or osmotic shock. addition, membrane-targeted, constitutively active (p110beta) was shown produce vivo products and activate JNK, while kinase-mutated protein showed no On basis these experiments, propose activity plays cells.