Influence of Furfural on anaerobic glycolytic kinetics of Saccharomyces cerevisiae in batch culture

摘要: Furfural was reduced to furfuryl alcohol by Saccaromyces cerevisiae with a yield of 0.97 +/- 0.01 mmol/ mmol causing lag phase in cell growth. In the presence 29 mM, furfural cell-mass on glucose decreased from 11.0 0.1 mg/mmol (0.06 0.0006 g/g) reference fermentation without 9.7 0.07 mg/ (0.05 0.0004 g/g), whereas ethanol increased 1.6 0.03 1.7 mmol/mmol. No glycerol excreted during reduction, and acetate production extended 1 h 5 furfural. Acetaldehyde pyruvate were reduction phase. Cell growth maintenance proportional consumption entire fermentation, ATP produced low reduction. These observations indicate that addition batch culture replication inhibiting activity (designated as replicative inactivation). The absence suggests acted an alternative redox sink oxidizing excess NADH formed biosynthesis. A mechanistic mathematical model developed described accurately based assumptions that: (i) NADH-dependent dehydrogenases had higher priority than dihydroxyacetone phosphate glycerol; (ii) caused inactivation replication. effect is discussed relation acetaldehyde formation. Inactivation modeled considering two populations within culture, both metabolically active, but only one replicating. kinetic description tool estimate transient fluxes carbon, NADH/NAD+ ATP/ADP.