A multi-Fc-species system for recombinant antibody production.
作者： Sandrine Moutel , Ahmed El Marjou , Ole Vielemeyer , Clément Nizak , Philippe Benaroch
关键词:
摘要: Genomic, transcriptomic and proteomic projects often suffer from a lack of functional validation creating strong demand for specific versatile antibodies. Antibody phage display represents an attractive approach to select rapidly in vitro the equivalent monoclonal antibodies, like single chain Fv inexpensive animal free way. However, so far, recombinant antibodies have not managed impose themselves as efficient alternatives natural We developed series vectors that allow one easily fuse Fc domains immunoglobulins, improving their sensitivity facilitating use. This enables fusion with human, mouse or rabbit given antibody is no longer restricted particular species. opens up unlimited multiplexing possibilities gives additional value also show this multi-Fc species production system can be applied cloned we converted widely used 9E10 anti-Myc-tag into human antibody. Altogether, new expression system, brings constant quality, unique versatility, will important broaden use both laboratory diagnosis
biomedcentral.com
springer.com
archives-ouvertes.fr
paperity.org参考文章(15)
Clément Nizak, Sandrine Moutel, Bruno Goud, Franck Perez, Selection and application of recombinant antibodies as sensors of rab protein conformation. Methods in Enzymology. ,vol. 403, pp. 135- 153 ,(2005) , 10.1016/S0076-6879(05)03012-0
Galit Denkberg, Yoram Reiter, Karl L Skorecki, Cyril J Cohen, Avital Lev, Maty Tzukerman, Patrick Chames, Hennie R Hoogenboom, Isolation and Characterization of Human Recombinant Antibodies Endowed with the Antigen-specific, Major Histocompatibility Complex-restricted Specificity of T Cells Directed toward the Widely Expressed Tumor T-cell Epitopes of the Telomerase Catalytic Subunit Cancer Research. ,vol. 62, pp. 3184- 3194 ,(2002)
Clément Nizak, Silvia Martin-Lluesma, Sandrine Moutel, Aurélien Roux, Thomas E. Kreis, Bruno Goud, Franck Perez, Recombinant Antibodies Against Subcellular Fractions Used to Track Endogenous Golgi Protein Dynamics in Vivo Traffic. ,vol. 4, pp. 739- 753 ,(2003) , 10.1034/J.1600-0854.2003.00132.X
David B. Powers, Peter Amersdorfer, Marie-Alix Poul, Ulrik B. Nielsen, M.Refaat Shalaby, Gregory P. Adams, Louis M. Weiner, James D. Marks, Expression of single-chain Fv-Fc fusions in Pichia pastoris Journal of Immunological Methods. ,vol. 251, pp. 123- 135 ,(2001) , 10.1016/S0022-1759(00)00290-8
Clément Nizak, Solange Monier, Elaine del Nery, Sandrine Moutel, Bruno Goud, Franck Perez, Recombinant antibodies to the small GTPase Rab6 as conformation sensors. Science. ,vol. 300, pp. 984- 987 ,(2003) , 10.1126/SCIENCE.1083911
Lidija Persic, Andy Roberts, Jane Wilton, Antonino Cattaneo, Andrew Bradbury, Hennie R Hoogenboom, An integrated vector system for the eukaryotic expression of antibodies or their fragments after selection from phage display libraries Gene. ,vol. 187, pp. 9- 18 ,(1997) , 10.1016/S0378-1119(96)00628-2
PATRICK FUCHS, FRANK BREITLING, MELVYN LITTLE, STEFAN DÜBEL, Primary Structure and Functional scFv Antibody Expression of an Antibody Against the Human Protooncogen c-myc Hybridoma. ,vol. 16, pp. 227- 233 ,(1997) , 10.1089/HYB.1997.16.227
Zoltán Konthur, Michael Hust, Stefan Dübel, Perspectives for systematic in vitro antibody generation Gene. ,vol. 364, pp. 19- 29 ,(2005) , 10.1016/J.GENE.2005.05.042
A. Dimitrov, M. Quesnoit, S. Moutel, I. Cantaloube, C. Pous, F. Perez, Detection of GTP-Tubulin Conformation in Vivo Reveals a Role for GTP Remnants in Microtubule Rescues Science. ,vol. 322, pp. 1353- 1356 ,(2008) , 10.1126/SCIENCE.1165401
Mathias Uhlén, Erik Björling, Charlotta Agaton, Cristina Al-Khalili Szigyarto, Bahram Amini, Elisabet Andersen, Ann-Catrin Andersson, Pia Angelidou, Anna Asplund, Caroline Asplund, Lisa Berglund, Kristina Bergström, Harry Brumer, Dijana Cerjan, Marica Ekström, Adila Elobeid, Cecilia Eriksson, Linn Fagerberg, Ronny Falk, Jenny Fall, Mattias Forsberg, Marcus Gry Björklund, Kristoffer Gumbel, Asif Halimi, Inga Hallin, Carl Hamsten, Marianne Hansson, My Hedhammar, Görel Hercules, Caroline Kampf, Karin Larsson, Mats Lindskog, Wald Lodewyckx, Jan Lund, Joakim Lundeberg, Kristina Magnusson, Erik Malm, Peter Nilsson, Jenny Ödling, Per Oksvold, Ingmarie Olsson, Emma Öster, Jenny Ottosson, Linda Paavilainen, Anja Persson, Rebecca Rimini, Johan Rockberg, Marcus Runeson, Åsa Sivertsson, Anna Sköllermo, Johanna Steen, Maria Stenvall, Fredrik Sterky, Sara Strömberg, Mårten Sundberg, Hanna Tegel, Samuel Tourle, Eva Wahlund, Annelie Waldén, Jinghong Wan, Henrik Wernérus, Joakim Westberg, Kenneth Wester, Ulla Wrethagen, Lan Lan Xu, Sophia Hober, Fredrik Pontén, A Human Protein Atlas for Normal and Cancer Tissues Based on Antibody Proteomics Molecular & Cellular Proteomics. ,vol. 4, pp. 1920- 1932 ,(2005) , 10.1074/MCP.M500279-MCP200