Down-regulation of RAG1 and RAG2 gene expression in PreB cells after functional immunoglobulin heavy chain rearrangement

摘要: Two waves of immunoglobulin gene rearrangements, first the heavy, then light chain loci form functional genes during B cell development. In mouse bone marrow differential surface expression B220 (CD45R), c-kit, CD25, and surrogate as well cycle status allows FACS separation cells in which these two rearrangements occur. The products recombination activating genes, RAG1 RAG2 are crucial for this rearrangement process. Here, we show that RAG is twice up- down-regulated, at transcriptional level RAG2, postranscriptional protein. Expression levels high D-->JH VH-->DJH rearranging proB preB-I cells, low preB expressing receptor on surface, again VL-->JL small preB-II cells. immature mRNA whereas protein maintained. Down-regulation after productive one heavy allele might be part mechanisms prevent further other allele.