Cross-linked polyethylenimine-tripolyphosphate nanoparticles for gene delivery.

摘要: The high transfection efficiency of polyethylenimine (PEI) makes it an attractive potential nonviral genetic vector for gene delivery and therapy. However, the highly positive charge PEI leads to cytotoxicity limits its application. To reduce PEI, we prepared anion-enriched nanoparticles that combined with tripolyphosphate (TPP). We then characterized PEI-TPP in terms size, zeta potential, Fourier-transform infrared (FTIR) spectra, assessed their efficiency, cytotoxicity, ability resist deoxyribonuclease (DNase) I digestion. cellular uptake phosphorylated internal ribosome entry site–enhanced green fluorescent protein C1 or FAM (fluorouracil, Adriamycin [doxorubicin] mitomycin)-labeled small interfering ribonucleic acids (siRNAs) was monitored by fluorescence microscopy confocal laser microscopy. transfected plasmid deoxyribonucleic acid (DNA) siRNA vitro 1.11- 4.20-fold higher particles (7.6% cross-linked) than at all N:P ratios (nitrogen phosphorus DNA) tested. cell viability different lines more 90% chosen PEI-TPP/DNA complexes. Moreover, resisted digestion DNase 2 hours. time-dependent absorption experiment showed 7.6% cross-linked were internalized 293T cells within 1 hour. In summary, effectively while conferring little no toxicity, thus have application delivery.