Flagellin Fusion Proteins as Adjuvants or Vaccines Induce Specific Immune Responses

摘要: Vaccination is the most efficient prophylaxis against a variety of infectious diseases. New vaccination strategies rely on incorporation effective adjuvants, which stimulate innate immune response and, in turn, activate adaptive response. It well established that flagellin induces inflammatory responses through activation antigen-presenting cells (APCs). In order to evaluate whether can serve as carrier for development adjuvants or vaccines, we prepared flagellin-enhanced green fluorescent protein (EGFP) fusion protein. Our results demonstrate flagellin-EGFP capable stimulating APCs, resulting maturation these and secretion proinflammatory cytokines. Furthermore, APCs pulsed with effectively process present EGFP antigens. More importantly, animals immunized developed specific anti-EGFP T-cell responses. contrast, recombinant was not able nor did it induce Thus, recombinant-flagellin proteins may be suitable carriers vaccines new boost diseases cancer.