Rumen microbial communities influence metabolic phenotypes in lambs

摘要: The rumen microbiota is an essential part of ruminants shaping their nutrition and health. Despite its importance, it not fully understood how various groups microbes affect host-microbe relationships functions. aim the study was to simultaneously explore metabolic phenotype lambs for identifying associations potential biomarkers digestive Twin lambs, separated in two after birth were exposed practices (isolation gavage with fluid protozoa or protozoa-depleted) that differentially restricted acquisition microbes. Rumen microbiota, fermentation parameters, digestibility growth monitored up 31 weeks age. Microbiota assembled isolation from other lacked had low bacterial archaeal diversity whereas affected. Exposure adult sheep increased independently presence. For archaea, Methanomassiliicoccales displaced Methanosphaera. Notwithstanding, induced differences functional traits such as significantly shaped community structure, notably Ruminococcaceae Lachnospiraceae lower 6 folds, Prevotellaceae by ~40%, Clostridiaceae Veillonellaceae higher 10 folds compared without protozoa. An orthogonal partial least squares-discriminant analysis urinary metabolome matched structure. Discriminant metabolites mainly involved amino acids protein pathways while a negative interaction observed between methylotrophic methanogens trimethylamine N-oxide. These results stress influence gut on animal show metabolomics monitoring microbial