Serum apolipoprotein A-1 quantification by LC–MS with a SILAC internal standard reveals reduced levels in smokers
作者： Qingqing Wang , Suhong Zhang , Lili Guo , Christine M Busch , Wenying Jian
DOI: 10.4155/BIO.15.195
关键词:
摘要: Background: Absolute quantification of protein biomarkers such as serum apolipoprotein A1 by both immunoassays and LC–MS can provide misleading results. Results: Recombinant ApoA-1 internal standard was prepared using stable isotope labeling amino acids in cell culture with [13C615N2]-lysine [13C915N1]-tyrosine human cells. A dilution method for validated levels analyzed 50 nonsmokers smokers. Conclusion: The concentration 169.4 mg/dl an 18.4% reduction to 138.2 assay will have clinical utility assessing effects smoking cessation therapeutic or dietary interventions high-risk populations.
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