Genotyping-Guided Discovery of Persiamycin A from Sponge-Associated Halophilic Streptomonospora sp. PA3

摘要: Microbial natural products have been a cornerstone of the pharmaceutical industry, but supply novel bioactive secondary metabolites has diminished due to extensive exploration most easily accessible sources, namely terrestrial Streptomyces species. The Persian Gulf is unique habitat for marine sponges, which contain diverse communities microorganisms including Actinobacteria. These exotic ecosystems may cradle rare actinomycetes with high potential produce metabolites. In this study, we harvested 12 different species sponges from two locations in and isolated 45 symbiotic assess their biodiversity sponge-microbe relationships. isolates were classified into Nocardiopsis (24 isolates), (17 isolates) genera (4 by 16S rRNA sequencing. Antibiotic activity tests revealed that culture extracts half displayed growth inhibitory effects against seven pathogenic bacteria. Next, identified five strains genetic aromatic polyketides genotyping ketosynthase genes responsible synthesis carbon scaffolds. combined data led us focus on Streptomonospora sp. PA3, since genus rarely examined its capacity Analysis discovery new polyketide denoted persiamycin A 1-hydroxy-4-methoxy-2-naphthoic acid. genome harbored gene clusters involved metabolism, tetracenomycin-type synthase pathway likely formation. work demonstrates use multivariate underexplored ecological niches guide drug process antibiotics anticancer agents.