Use of monoclonal antibodies to culture rat proximal tubule cells.

摘要: Current renal cell culture techniques are limited by either a low yield of cells or heterogeneity types. We have used monoclonal antibodies to microvillus membrane proteins isolate and pure population proximal tubule cells. The were characterized as phase microscopy, enzyme histochemistry for alkaline phosphatase, butyrate esterase, gamma-glutamyltransferase, electron specific reactivity with variety Growth over 2-7 days yielded numbers up 1,000-fold greater than obtained single microdissection. Dome formation was observed, suggesting intact fluid transport. In addition, Na+-H+ exchange Na+-dependent D-hexose transport, known transport processes the tubule, demonstrated microfluorimetry methyl-alpha-D-glucopyranoside uptake, respectively. Our results indicate that large homogeneous, cultured rat maintain characteristics in vivo can be using antibody technique isolation.