Isolation of a lipopolysaccharide (LPS)-resistant mutant, with defective LPS binding, of cultured macrophage-like cells.
作者： S Hara-Kuge , F Amano , M Nishijima , Y Akamatsu
DOI: 10.1016/S0021-9258(19)39191-4
关键词:
摘要: Lipopolysaccharide (LPS)-resistant mutants which did not respond to LPS were isolated from a macrophage-like mouse cell line, J774.1. Unlike the parental J774.1 cells, these grew even in added medium as well normal growth without any morphological changes. Assay of 125I-LPS binding monolayers revealed that one LPS-resistant (LR-9) was strikingly defective LPS-binding activity. Scatchard plot showed LR-9 cells lacked high affinity sites present The inhibited by addition excess unlabeled LPS, lipid A, IVA (tetraacyl-beta(1'-6)-linked D-glucosamine disaccharide-1,4'-bisphosphate), and X (2,3-diacylglucosamine 1-phosphate) sensitive proteinase K. enhanced O2- generation release arachidonic acid but cells. Other stimulants such zymosan 12-O-tetradecanoylphorbol 13-acetate, however, induced LPS-photocross-linked assay allowed identification 65- 55-kDa proteins membrane fraction Both bands detectable disappeared competing with or X. These results show both two might relate specific receptor for LPS.
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