Membrane changes in lipopolysaccharide-stimulated murine B lymphocytes associated with cell activation

摘要: Abstract The lateral diffusion of the fluorescent lipid analog 3,3′-dioctadecylindocarbocyanine iodide (DiI) was measured in membranes murine B lymphocytes treated with cell mitogen lipopolysaccharide (LPS). mobility DiI, as by fluorescence photobleaching recovery (FPR) techniques, temperature-dependent a value 6.1 · 10−9 cm2 s−1 at 37°C. Untreated cells exhibited this coefficient over 72 h culture. In contrast, DiI decreased to 2.0 cm2s−1 37°C LPS-stimulated 24 following LPS exposure. Interestingly, not accompanied any change surface immunoglobulin which remained essentially unchanged 3.6-4.3 10−11 h. To determine whether effects on were due insertion into plasma membrane, we examined TRITC-LPS from LPS-responsive Balb/c and C3Heb/FeJ mice hypo-responsive C3H/HeJ mice. more than 50% On lymphocytes, there no or throughout incubation period. These data indicate that lymphocyte membrane compositions is altered LPS-activated lymphoblasts probes does result perturbation bilayer. Further, similarities between suggest most molecules interact non-specifically membranes, presumably acyl chain A moiety.