Highly stable and sensitive glucose biosensor based on covalently assembled high density Au nanostructures.

摘要: Abstract We describe the development of a highly stable and sensitive glucose biosensor based on nanohybrid materials derived from gold nanoparticles (AuNPs) multi-walled carbon nanotubes (MWCNT). The biosensing platform was developed by using layer-by-layer (LBL) self-assembly enzyme oxidase (GOx). A high density AuNPs MWCNT nanocomposite were constructed alternate self assembly thiol functionalized MWCNTs AuNPs, followed chemisoption GOx. surface morphology multilayered AuNPs/MWCNT structure characterized field emission-scanning electron microscope (FE-SEM), coverage investigated cyclic voltammetry (CV), showing that 5 layers achieves maximum particle electrode. immobilization GOx monitored electrochemical impedance spectroscopy (EIS). CV amperometry methods used to study oxidation at physiological pH 7.4. Au electrode modified with five composites exhibited an excellent electrocatalytic activity towards glucose, which presents wide liner range 20 μM 10 mM, sensitivity 19.27 μA mM −1  cm −2 . detection limit present found be 2.3 μM (S/N = 3). In addition, resulting showed faster amperometric current response (within 3 s) low apparent Michaelis–Menten constant ( K m app ) Our shows decorated is promising material for construction biosensors.