High-Resolution Autoradiography of H3-Estradiol with Unfixed, Unembedded 1.0µ Freeze-Dried Frozen Sections

摘要: The autoradiographic localization of labeled compounds imposes severe limitations on the selection procedures to which tissue is subjected in process. Unfortunately, this fact has not been fully recognized, and numerous studies questionable value have carried out with unsuitable methods. Since precise purpose high resolution autoradiography, all steps procedure are likely cause translocation label must be rigidly controlled or excluded. successful H3-thymidine, incorporated into nuclear DNA, led many people regard a valid autoradiography thymidine as equally study diffusible compounds.