Expression of an enzymatically active parasite molecule in Escherichia coli: Schistosoma japonicum glutathione S-transferase.
作者： Michael R. Rubira , Richard J. Simpson , Kathy M. Davern , Wilfred U. Tiu , Philip G. Board
DOI: 10.1016/0166-6851(88)90044-8
关键词:
摘要: The NH2-terminal amino acid sequence of the Mr 26 000 glutathione S-transferase (EC 2.5.1.18) Schistosoma japonicum (Sj26) has been deduced by RNA and protein analysis. Using this information, a bacterial plasmid constructed that directs synthesis entire Sj26 molecule in Escherichia coli. Recombinant exhibits activity can be readily purified from bacteria one-step procedure under non-denaturing conditions. availability recombinant essentially unlimited quantities will aid its assessment as candidate vaccine schistosomiasis could eventually lead to rational design drug targetted on schistosome S-transferases.
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