The effect of hOGG1 and glutathione peroxidase I genotypes and 3p chromosomal loss on 8-hydroxydeoxyguanosine levels in lung cancer.

摘要: Polymorphic genes for the peroxide scavenger glutathione peroxidase I (GPX1) and 8-hydroxydeoxyguanosine (8-OHdG) DNA glycosylase/apurinic (AP) lyase (hOGG1) map to loci on chromosome 3p which are subject frequent loss of heterozygosity (LOH) in lung tumours. Levels pro-mutagenic, oxidative lesion 8-OHdG, were measured 37 paired normal tumorous specimens using HPLC with electrochemical detection. Lung tumours also analysed LOH by fluorescent PCR Genescan analysis. No significant difference was observed between 8-OHdG levels tumour [7.7 +/- 6.7 (mean SE) 8-OHdG/10(6) 2'-deoxyguanosine (dG)] (8.1 8.8 dG) tissue. Adduct tissue not associated constitutive hOGG1 genotype although there a trend towards lower individuals possessing ALA6 GPX1 polymorphism. exhibiting (40%) contained higher adducts (10.9 2.6 (P = 0.05) enzyme activity [45.5 nmol (GSH)/min/mg] 0.09) when compared without at these sites (5.55 0.87 dG 63.6 GSH/min/mg, respectively). In conclusion, appear have compromised defence mechanisms as reduced elevated this may affect prognosis cancer patients.