Development of an enzymatic fiber-optic biosensor for detection of halogenated hydrocarbons.

摘要: An enzyme-based biosensor was developed by co-immobilization of purified enzyme haloalkane dehalogenase (EC 3.8.1.5) and a fluorescence pH indicator on the tip an optical fiber. Haloalkane catalyzes hydrolytic dehalogenation halogenated aliphatic hydrocarbons, which is accompanied change influencing indicator. The sensitivity several fluorescent dyes evaluated. selected 5(6)-carboxyfluorescein conjugated with bovine serum albumin its reaction tested under different immobilization conditions. prepared cross-linking conjugate in tandem using glutaraldehyde vapor. biosensor, stored for 24 h 50 mM phosphate buffer (pH 7.5) prior to measurement, used after 15 min equilibration, compound added, response monitored 30 min. Calibration 1,2-dibromoethane 3-chloro-2-(chloromethyl)-1-propene showed excellent linear dependence, detection limits 0.133 0.014 mM, respectively. This provides new tool continuous situ monitoring environmental pollutants.