Light microscopical localization of enzymes by means of cerium-based methods. I. Detection of acid phosphatase by a new cerium-lead-technique (Ce-Pb-method).

摘要: Cerium-III-ions are more and used as capturing reagent opaque marker for the electron microscopic localization of a number H2O2-generating enzymes well phosphohydrolases. Contrary to its advantages over common lead methods in histochemical detection enzyme activities at level, cerium-based proved be failure light investigations. Therefore, our method ultrahistochemical acid phosphatase was developed further observations level. The principle that new Ce-Pb-method is conversion not visible cerium phosphate into by secondary capture alkaline citrate. Finally, can visualized sulfide section. finally proposed manner compared with showed range advantages. Because fact, recommended broader use histochemistry, e.g. investigation parallel preparations.