A Highly Sensitive Fluorescence Probe for Fast Thiol-Quantification Assay of Glutathione Reductase†

作者: Long Yi , Heyang Li , Lu Sun , Liangliang Liu , Caihong Zhang

DOI: 10.1002/ANIE.200805693

关键词:

摘要: Fast detection of cellular thiols in aqueous medium was achieved using a newly developed fluorescence probe (see picture). Based on this probe, high-throughput assay for glutathione reductase developed.

参考文章(34)
S Shigeoka, T Onishi, Y Nakano, S Kitaoka, Characterization and physiological function of glutathione reductase in Euglena gracilis z. Biochemical Journal. ,vol. 242, pp. 511- 515 ,(1987) , 10.1042/BJ2420511
R. Heiner Schirmer, Joachim G. Müller, R. Luise Krauth-Siegel, Inhibitoren von Disulfid‐Reduktasen als Chemotherapeutica – Design von Wirkstoffen gegen die Chagas‐Krankheit und gegen die Malaria Angewandte Chemie. ,vol. 107, pp. 153- 166 ,(1995) , 10.1002/ANGE.19951070204
Meng Zhang, Mengxiao Yu, Fuyou Li, Minwei Zhu, Manyu Li, Yanhong Gao, Lei Li, Zhiqiang Liu, Jianping Zhang, Dengqing Zhang, Tao Yi, Chunhui Huang, A Highly Selective Fluorescence Turn-on Sensor for Cysteine/Homocysteine and Its Application in Bioimaging Journal of the American Chemical Society. ,vol. 129, pp. 10322- 10323 ,(2007) , 10.1021/JA073140I
Holger Bauer, Karin Fritz-Wolf, Andreas Winzer, Sebastian Kühner, Susan Little, Vanessa Yardley, Hervé Vezin, Bruce Palfey, R. Heiner Schirmer, Elisabeth Davioud-Charvet, A Fluoro Analogue of the Menadione Derivative 6-[2‘-(3‘-Methyl)-1‘,4‘-naphthoquinolyl]hexanoic Acid Is a Suicide Substrate of Glutathione Reductase. Crystal Structure of the Alkylated Human Enzyme† Journal of the American Chemical Society. ,vol. 128, pp. 10784- 10794 ,(2006) , 10.1021/JA061155V
Berivan Tandoğan, N. Nuray Ulusu, The inhibition kinetics of yeast glutathione reductase by some metal ions. Journal of Enzyme Inhibition and Medicinal Chemistry. ,vol. 22, pp. 489- 495 ,(2007) , 10.1080/14756360601162147
Yanyan Fu, Hongxiang Li, Wenping Hu, Daoben Zhu, Fluorescence probes for thiol-containing amino acids and peptides in aqueous solution. Chemical Communications. pp. 3189- 3191 ,(2005) , 10.1039/B503772G
Julia Guy, Karine Caron, Stéphane Dufresne, Stephen W. Michnick, Skene, Jeffrey W. Keillor, Convergent preparation and photophysical characterization of dimaleimide dansyl fluorogens : Elucidation of the maleimide fluorescence quenching mechanism Journal of the American Chemical Society. ,vol. 129, pp. 11969- 11977 ,(2007) , 10.1021/JA0738125
Runa Njälsson, Svante Norgren, Physiological and pathological aspects of GSH metabolism. Acta Paediatrica. ,vol. 94, pp. 132- 137 ,(2005) , 10.1111/J.1651-2227.2005.TB01878.X